human hnscc cell lines scc25  (ATCC)

Journal: MedComm

Article Title: Comprehensive Integrated Single‐Cell and Spatial Transcriptomics Unveil the Dynamic Landscape of Betel Nut‐Associated Oral Mucosal Carcinogenesis and Its Tumor Microenvironment

doi: 10.1002/mco2.70796

Figure Lengend Snippet: Arecoline enhances the migratory and invasive potential of LAMC2 + OSCC cells. (A) Representative immunohistochemical staining images showing LAMC2 expression in OSCC tissues from betel nut chewers and nonchewers. The box‐and‐whisker plot on the right summarizes LAMC2 expression levels; each dot represents an individual clinical specimen (**** p < 0.0001 by Student's t ‐test). (B) Western blot images showing the expression of LAMC2, EMT‐associated markers (N‐cadherin, E‐cadherin, and Vimentin), and GAPDH in OSCC cell lines SCC25 and SCC9 under control and arecoline‐treated conditions. (C) Representative Transwell assay images illustrating migratory and invasive capacities of SCC25 and SCC9 cells in control and arecoline‐treated groups. (D) Bar plots showing the quantification of migration and invasion assays for SCC25 and SCC9 cells under control and arecoline‐treated conditions ( n = 3, *** p < 0.001, **** p < 0.0001 by Student's t ‐test).

Article Snippet: SCC9 and SCC25 cells were obtained from the American Type Culture Collection.

Techniques: Immunohistochemical staining, Staining, Expressing, Whisker Assay, Western Blot, Control, Transwell Assay, Migration

Journal: Cancer Cell International

Article Title: LIS1 mediated Schwann cell reprogramming enhances perineural invasion by activating the serine/NMDAR/AKT signaling pathway in head and neck squamous carcinoma

doi: 10.1186/s12935-026-04243-0

Figure Lengend Snippet: LIS1 increases the migration, invasion, and PNI capability of HNSCC cells. ( A ) Wound healing assay of LIS1 overexpression or LIS1 knockdown in SCC25 cells. ( B ) Transwell assay of LIS1 overexpression or LIS1 knockdown in SCC25 cells. ( C ) Representative images of the in vitro PNI model for LIS1 overexpression or LIS1 knockdown in SCC25 cells. ( D ) Microfluidic chip pattern diagram and representative images of LIS1 overexpression or LIS1 knockdown in SCC25 cells co-cultured with SCs in the microfluidic chip. Data are represented as mean ± SEM, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: Human HNSCC cell line (SCC25) and human Schwann cells (sNF96.2) were acquired from the American Type Culture Collection (ATCC, Manassas, VA, USA) in 2023.

Techniques: Migration, Wound Healing Assay, Over Expression, Knockdown, Transwell Assay, In Vitro, Cell Culture

Journal: Cancer Cell International

Article Title: LIS1 mediated Schwann cell reprogramming enhances perineural invasion by activating the serine/NMDAR/AKT signaling pathway in head and neck squamous carcinoma

doi: 10.1186/s12935-026-04243-0

Figure Lengend Snippet: LIS1 overexpression of HNSCC cells increases Schwann cells activity. ( A ) The pattern diagram of HNSCC cells co-cultured with SCs. ( B ) Transwell assay of sNF96.2 cells co-cultured with SCC25 cells. ( C ) The mRNA expression of PNI-related factors (BDNF, GDNF, NGF, MMP2, and MMP9) in sNF96.2 cells co-cultured with SCC25 cells were analyzed by RT-qPCR. ( D ) IF double staining was performed to detect the expression of S100 and GFAP in the sNF96.2 cells co-cultured with SCC25 cells. Data are represented as mean ± SEM, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: Human HNSCC cell line (SCC25) and human Schwann cells (sNF96.2) were acquired from the American Type Culture Collection (ATCC, Manassas, VA, USA) in 2023.

Techniques: Over Expression, Activity Assay, Cell Culture, Transwell Assay, Expressing, Quantitative RT-PCR, Double Staining

Journal: Cancer Cell International

Article Title: LIS1 mediated Schwann cell reprogramming enhances perineural invasion by activating the serine/NMDAR/AKT signaling pathway in head and neck squamous carcinoma

doi: 10.1186/s12935-026-04243-0

Figure Lengend Snippet: LIS1 overexpression activates the serine signaling pathway to promote the PNI in HNSCC cells. ( A ) The mRNA expression of PHGDH, PSAT1, and PSPH in SCC25 cells were analyzed by RT-qPCR. ( B ) Analysis of the relative intracellular serine levels in SCC25 cells. ( C ) The protein level of PHGDH, PSAT1, and PSPH in SCC25 cells were analyzed by WB. ( D ) Migration and invasion assay of shLIS1 group after exogenous serine stimulation of SCC25 cells and quantitative analysis. ( E ) Representative images of the in vitro PNI model for shLIS1 group after exogenous serine stimulation of SCC25 cells and the analysis of neural invasion index. ( F ) Representative images of the microfluidic chip for shLIS1 group after exogenous serine stimulation of SCC25 cells and analysis of migration distance. Data are represented as mean ± SEM, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: Human HNSCC cell line (SCC25) and human Schwann cells (sNF96.2) were acquired from the American Type Culture Collection (ATCC, Manassas, VA, USA) in 2023.

Techniques: Over Expression, Expressing, Quantitative RT-PCR, Migration, Invasion Assay, In Vitro